Journal: Current biology : CB

Article Title: A critical role for thermosensation in host seeking by skin-penetrating nematodes

doi: 10.1016/j.cub.2018.05.063

Figure Lengend Snippet: QUANTIFICATION AND STATISTICAL ANALYSIS

Article Snippet: Recombinant DNA Sr-eef-1A pro ::Cas9::Ss-era-1 3’UTR Dr. James Lok; [ 48 ] pPV540; Strongyloides codon-optimized Cas9 Sr-U6 pro ::Ss-tax-4-sgRNA-1::Sr-U6 3’UTR [ 48 ] pMLC47; sgRNA for Ss-tax-4 5’HA:: Ss-act-2 pro ::mRFPmars::Ss-era-1 3’UTR::3’HA This paper pEY11; HDR construct for Ss- tax-4 Sr-tax-4 pro ::GFP:: Ss-era-1 3’UTR This paper pMC41 Software and Algorithms GraphPad Prism 6 GraphPad http://ww.graphpad.com Zeiss AxioVision 4.8 Carl Zeiss https://www.zeiss.com/microscopy/us/products/microscope-software/axiovision.html Fiji [ 61 ] https://fiji.sc/ PAST 3.19 [ 62 ] https://folk.uio.no/ohammer/past/ MATLAB R2107B MathWorks https://www.mathworks.com/products/matlab.html Mightex Cam Demo v1.2.1 Mightex Systems http://www.mightexsystems.com/ FTC100D TEC controller Accuthermo Technology http://www.accuthermo.com/ Other 3% thermotaxis agar (17 g agar, 3 g NaCl, 1 mL 1M CaCl 2 , 1 mL 1M MgSO 4 , 25 mL 1M KH 2 PO 4 pH 6.0, dH 2 O to 1 L) [ 17 ] Thermotaxis agar M9 solution (3 g KH 2 PO 4 , 6 g Na 2 HPO 4 , 5 g NaCl, 1 mL 1 M MgSO 4 , dH 2 O to 1 L) [ 59 ] M9 solution BU saline (7.10 g Na 2 HPO 4 , 2.99 g KH 2 PO 4 , 4.09 g NaCl, dH 2 O to 1 L) [ 57 ] BU saline 2% NGM plates (3 g NaCl, 2.5 g Bacto Peptone, 20 g agar, 1 mL 5mg/mL cholesterol, 1 mL 1M CaCl 2 , 1 mL 1M MgSO 4 , 25 mL 1M KPO 4 pH 6.0, dH 2 O to 1 L) [ 59 ] 2% NGM plates USB-DAQ device LabJack Corp U3-LV Isotemp Refrigerated/Heated Water Bath Fisher Scientific 13-874-126 Commercial water block Swiftech MCW820 5-megapixel CMOS camera Mightex Systems BTE-B050-U Zoom lenses Kowa America Corp LMZ69M H-bridge amplifier Accuthermo Technology FTX300 TEC temperature controller Accuthermo Technology FTC100D Peltier element Multicomp MCTE1-19913L-S 22x22 cm Bioassay dishes Corning 431301 Wacom Intuos Art touch tablet and stylus Wacom CTH-690AK Open in a separate window QUANTIFICATION AND STATISTICAL ANALYSIS

Techniques: Recombinant, CRISPR, Sequencing, Construct, Software, Blocking Assay